Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice

Jamiyansuren Jambaldorj, Satoshi Makino, Batmunkh Munkhbat, Gen Tamiya

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II-mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N- TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N- Taf1 mRNA in mouse tissues and cell lines. As well as the human N- TAF1, the N- Taf1 showed limited expression in the brain and neuroblastoma, whereas Taf1 expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N- Taf1 showed sustained expression. Our result suggests that the N- Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalBiochemical and biophysical research communications
Volume425
Issue number2
DOIs
Publication statusPublished - 2012 Aug 24
Externally publishedYes

Keywords

  • Neuron-specific isoform
  • TAF1
  • Transcription factor
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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