Suspension culture of Nicotiana tabacum cells in a rotary‐drum bioreactor

Naomi Shibasaki, Kenji Hirose, Toshikuni Yonemoto, Teiriki Tadaki

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Suspension cultures of Nicotiana tabacum (tobacco) were studied using a rotary‐drum bioreactor and the effects of various operating factors on culture growth were investigated. Conditions of oxygen concentration, aeration and drum rotation rates required for optimum culture growth rate were determined. The results were compared with those obtained in an air‐lift bioreactor with a draft tube. The culture growth rates were higher in the rotary‐drum bioreactor than in the air‐lift bioreactor under the same conditions of aeration. The rotary‐drum bioreactor was superior to the air‐lift bioreactor in respect of oxygen mass transfer and the level of shear damage to N. tabacum cells.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalJournal of Chemical Technology & Biotechnology
Volume53
Issue number4
DOIs
Publication statusPublished - 1992

Keywords

  • Nicotiana tabacum
  • air‐lift bioreactor
  • roller bottle
  • rotary‐drum bioreactor
  • suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

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