Susceptibility difference between methicillin-susceptible and methicillin-resistant Staphylococcus aureus to a bovine myeloid antimicrobial peptide (BMAP-28)

Shiaki Takagi, Junko Nishimura, Lanlan Bai, Hikaru Miyagi, Kengo Kuroda, Shunji Hayashi, Hiroshi Yoneyama, Tasuke Ando, Hiroshi Isogai, Emiko Isogai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A bovine myeloid antimicrobial peptide antimicrobial peptide (BMAP-28) is a member of the cathelicidin family and acts as a component of innate immunity. There are few reports of susceptibility difference of methicillin-resistant Staphylococcus aureus (MRSA) and susceptible strains (MSSA) against BMAP-28. This study aims to clarify how a few amino acid substitutions of BMAP-28 are related to its antimicrobial activity using four analog peptides of BMAP-28. We also compared cellular fatty acid components of MSSA and MRSA using gas chromatography. We found that a few amino acid substitutions of BMAP-28 do not change antimicrobial activity. It was also revealed that the percentage of cis-11-eicosenoic acid in total detected fatty acids of MRSA was significantly higher than that of MSSA. In addition, the percentage of palmitic acid in total detected fatty acids of MRSA tended to be lower than that of MSSA. Our results will provide new information to deal with the question of differences in bacterial susceptibility against BMAP-28.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalAnimal Science Journal
Volume85
Issue number2
DOIs
Publication statusPublished - 2014 Feb

Keywords

  • Amino acid substitution
  • Antimicrobial peptide
  • Drug-resistant bacteria
  • Fatty acid component
  • Susceptibility

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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