Survival of the aerobic denitrifier pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions

Morio Miyahara, Sang Wan Kim, Shengmin Zhou, Shinya Fushinobu, Takeshi Yamada, Wakako Ikeda-Ohtsubo, Akira Watanabe, Keisuke Miyauchi, Ginro Endo, Takayoshi Wakagi, Hirofumi Shoun

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The aerobic denitrifier Pseudomonas stutzeri TR2 (strain TR2) has the potential to reduce nitrous oxide emissions during the wastewater treatment process. In this application, it is important to find the best competitive survival conditions for strain TR2 in complex ecosystems. To that end, we examined cocultures of strain TR2 with activated sludge via five passage cultures in a medium derived from treated piggery wastewater that contained a high concentration of ammonium. The results are as follows: (i) The medium supported the proliferation of strain TR2 (P. stutzeri strains) under denitrifying conditions. (ii) Nitrite was a better denitrification substrate than nitrate for TR2 survival. (iii) Strain TR2 also demonstrated strong survival even under aerobic conditions. This suggests that strain TR2 is effectively augmented to the wastewater treatment process, aiding in ammoniumnitrogen removal and reducing nitrous oxide production with a partial nitrification technique in which nitrite accumulates.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number3
DOIs
Publication statusPublished - 2012

Keywords

  • Bacterial population shift
  • Denitrification
  • Passage culture
  • Pseudomonas stutzeri
  • Respiration

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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