Surprisingly high correlation between early and late stages in non-two-state protein folding

Kiyoto Kamagata, Kunihiro Kuwajima

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We collected quantitative kinetic data on early and late stages of folding in non-two-state proteins from the literature, and studied the relationship between the kinetics of the two stages. There was a surprisingly high correlation between the rate constants of these stages. The correlation coefficient of the logarithmic rate constants was as high as 0.97, which could not be caused by chance. We also studied relationships of the logarithmic rate constants of the two stages with native three-dimensional structures represented by the residue-residue contact map. There were again surprisingly high correlations between the logarithmic rate constants and the number of non-local contact clusters obtained from the contact maps. Because the number of non-local contact clusters represents overall arrangement of substructures in a native protein, the results strongly suggested the importance of the arrangement of the substructures for the kinetics of both early and late stages of protein folding.

Original languageEnglish
Pages (from-to)1647-1654
Number of pages8
JournalJournal of Molecular Biology
Volume357
Issue number5
DOIs
Publication statusPublished - 2006 Apr 14
Externally publishedYes

Keywords

  • Backbone topology
  • Folding intermediate
  • Folding kinetics
  • Non-two-state protein
  • Protein folding

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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