Surface structures and osteoblast response of hydrothermally produced CaTiO 3 thin film on Ti-13Nb-13Zr alloy

Jin Woo Park, Yusuke Tustusmi, Chong Soo Lee, Chan Hee Park, Youn Jeong Kim, Je Hee Jang, Dongwoo Khang, Yeon Min Im, Hisashi Doi, Naoyuki Nomura, Takao Hanawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO 3 layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO 3 layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.

Original languageEnglish
Pages (from-to)7856-7863
Number of pages8
JournalApplied Surface Science
Volume257
Issue number17
DOIs
Publication statusPublished - 2011 Jun 15
Externally publishedYes

Keywords

  • Calcium ion
  • Hydrothermal treatment
  • Osteoblast
  • Surface hydrophilicity
  • Ti-13Nb-13Zr alloy

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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