Surface modification by grafting with biocompatible 2-methacryloyloxyethyl phosphorylcholine for microfluidic devices

James Sibarani; Tomohiro Konno; Madoka Takai; Kazuhiko Ishihara

doi:10.4028/0-87849-436-7.789

Surface modification by grafting with biocompatible 2-methacryloyloxyethyl phosphorylcholine for microfluidic devices

James Sibarani, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter) and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)-grafted chains were 320 kDa and 95 ±14 nm, respectively. Characterizations of the poly(MPC) modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy, atomic force microscope. Protein adsorption resistance of these modified surfaces was then investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These poly(MPC)-modified surfaces effectively reduced protein adsorption.

Original language	English
Pages (from-to)	789-792
Number of pages	4
Journal	Key Engineering Materials
Volume	342-343
DOIs	https://doi.org/10.4028/0-87849-436-7.789
Publication status	Published - 2007 Jan 1
Externally published	Yes

Keywords

High S/N ratio
Microchannel
Nonspecific protein adsorption
Poly(MPC) brush
Surface modification

ASJC Scopus subject areas

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

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10.4028/0-87849-436-7.789

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title = "Surface modification by grafting with biocompatible 2-methacryloyloxyethyl phosphorylcholine for microfluidic devices",

abstract = "Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter) and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)-grafted chains were 320 kDa and 95 ±14 nm, respectively. Characterizations of the poly(MPC) modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy, atomic force microscope. Protein adsorption resistance of these modified surfaces was then investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These poly(MPC)-modified surfaces effectively reduced protein adsorption.",

keywords = "High S/N ratio, Microchannel, Nonspecific protein adsorption, Poly(MPC) brush, Surface modification",

author = "James Sibarani and Tomohiro Konno and Madoka Takai and Kazuhiko Ishihara",

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doi = "10.4028/0-87849-436-7.789",

language = "English",

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T1 - Surface modification by grafting with biocompatible 2-methacryloyloxyethyl phosphorylcholine for microfluidic devices

AU - Sibarani, James

AU - Konno, Tomohiro

AU - Takai, Madoka

AU - Ishihara, Kazuhiko

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter) and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)-grafted chains were 320 kDa and 95 ±14 nm, respectively. Characterizations of the poly(MPC) modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy, atomic force microscope. Protein adsorption resistance of these modified surfaces was then investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These poly(MPC)-modified surfaces effectively reduced protein adsorption.

AB - Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter) and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)-grafted chains were 320 kDa and 95 ±14 nm, respectively. Characterizations of the poly(MPC) modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy, atomic force microscope. Protein adsorption resistance of these modified surfaces was then investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These poly(MPC)-modified surfaces effectively reduced protein adsorption.

KW - High S/N ratio

KW - Microchannel

KW - Nonspecific protein adsorption

KW - Poly(MPC) brush

KW - Surface modification

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JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

Surface modification by grafting with biocompatible 2-methacryloyloxyethyl phosphorylcholine for microfluidic devices

Abstract

Keywords

ASJC Scopus subject areas

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