Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles

Jun Ando, Takumasa Sekiya, Den Ka, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, Katsumasa Fujita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) spectroscopy to perform highly-sensitive and selective drug imaging in live cells. Gold nanoparticles are introduced in lysosomes through endocytosis as SERS agents, and the alkyne-tagged drugs are subsequently administered in cells. Raman microscopic observation reveals the arrival of drug in lysosome through enhanced Raman signal of alkyne. Since the peak of alkyne appears in Raman-silent region of biomolecules, selective detection of drugs is possible without background signal of endogenous molecules. From endocytosed gold nanoparticles in living HeLa cells, we observed distinct Raman signal from alkyne-tagged inhibitor of lysosomal enzyme.

Original languageEnglish
Title of host publicationVisualizing and Quantifying Drug Distribution in Tissue
EditorsConor L. Evans, Kin Foong Chan
PublisherSPIE
ISBN (Electronic)9781510605336
DOIs
Publication statusPublished - 2017
Externally publishedYes
EventVisualizing and Quantifying Drug Distribution in Tissue - San Francisco, United States
Duration: 2017 Jan 28 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10046
ISSN (Print)1605-7422

Conference

ConferenceVisualizing and Quantifying Drug Distribution in Tissue
CountryUnited States
CitySan Francisco
Period17/1/28 → …

Keywords

  • alkyne-tag
  • gold nanoparticle
  • live cell
  • Raman imaging
  • small molecule
  • Surface-enhanced Raman scattering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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