Suramin and reactive blue 2 are antagonists for a newly identified purinoceptor on rat megakaryocyte

Hisayuki Uneyama, Chikako Uneyama, Satoru Ebihara, Norio Akaike

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The effects of purinoceptor antagonists on ATP‐induced oscillatory K+‐currents in rat isolated megakaryocytes were investigated. Both reactive blue‐2 (RB‐2), a selective antagonist of the P2Y purinoceptor, at concentrations of 0.3–10 μm and suramin, a non‐selective P2 purinoceptor antagonist, at 1–30 μm blocked the ATP‐induced oscillation in a concentration‐dependent manner. RB‐2 and suramin also blocked the ADP‐induced K+‐current oscillation at the same concentration range as in the case of ATP. However, both suramin and RB‐2 had no effect on thrombin‐ and inositol 1,4,5‐trisphosphate (IP3)‐induced K+ current oscillation, indicating that they act as specific purinoceptor antagonists. Thus, the purinoceptors on megakaryocytes show the properties of the P2 subtype according to their blockade by antagonists. 1994 British Pharmacological Society

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalBritish Journal of Pharmacology
Volume111
Issue number1
DOIs
Publication statusPublished - 1994 Jan
Externally publishedYes

Keywords

  • Megakaryocytes
  • purinoceptors
  • reactive blue 2
  • suramin

ASJC Scopus subject areas

  • Pharmacology

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