Suppressive potential of Paenibacillus strains isolated from the tomato phyllosphere against Fusarium crown and root rot of tomato

Ikuo Sato, Shigenobu Yoshida, Yutaka Iwamoto, Masataka Aino, Mitsuro Hyakumachi, Masafumi Shimizu, Hideki Takahashi, Sugihiro Ando, Seiya Tsushima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The suppressive potentials of Bacillus and Paenibacillus strains isolated from the tomato phyllosphere were investigated to obtain new biocontrol candidates against Fusarium crown and root rot of tomato. The suppressive activities of 20 bacterial strains belonging to these genera were examined using seedlings and potted tomato plants, and two Paenibacillus strains (12HD2 and 42NP7) were selected as biocontrol candidates against the disease. These two strains suppressed the disease in the field experiment. Scanning electron microscopy revealed that the treated bacterial cells colonized the root surface, and when the roots of the seedlings were treated with strain 42NP7 cells, the cell population was maintained on the roots for at least for 4 weeks. Although the bacterial strains had no direct antifungal activity against the causal pathogen in vitro, an increase was observed in the antifungal activities of acetone extracts from tomato roots treated with the cells of both bacterial strains. Furthermore, RT-PCR analysis verified that the expression of defense-related genes was induced in both the roots and leaves of seedlings treated with the bacterial cells. Thus, the root-colonized cells of the two Paenibacillus strains were considered to induce resistance in tomato plants, which resulted in the suppression of the disease.

Original languageEnglish
Pages (from-to)168-177
Number of pages10
JournalMicrobes and environments
Volume29
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Fusarium crown and root rot
  • Fusarium oxysporum f. sp
  • Induced resistance
  • Paenibacillus
  • Phyllosphere bacteria
  • Radicis-lycopersici

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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