Suppression of the antigen-stimulated RBL-2H3 mast cell activation by artekeiskeanol A

Jangja Hong, Hirokazu Sasaki, Noriyasu Hirasawa, Kenji Ishihara, Jong Hwan Kwak, Okpyo Zee, Francis J. Schmitz, Toshio Seyama, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Effects of artekeiskeanol A, a newly isolated coumarin derivative from Artemisa keiskeana Miq. (Compositae), the extract of which is used for treatment of rheumatoid arthritis as a folk medicine, on the antigen-induced activation of RBL-2H3 cells were examined. RBL-2H3 cells were sensitized with dinitrophenol (DNP)-specific IgE, and then stimulated with the antigen DNP-conjugated human serum albumin (DNPHSA). Artekeiskeanol A at 10 to 100μM inhibited the antigen-induced degranulation in a concentration-dependent manner, the IC 50 value being 38.0±0.2μM. Degranulation induced by thapsigargin or A23187 also was inhibited by artekeiskeanol A at 10 to 100μM. The antigen-induced increase in the levels of mRNA for tumor necrosis factor (TNF)-α and interleukin (IL)-13 and phosphorylations of Akt, p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK) and p44/42 MAPK were also suppressed by artekeiskeanol A. Our findings suggested that the effectiveness of the extract of A.keiskeana might partly be due to the inhibition of mast cell activation by artekeiskeanol A.

Original languageEnglish
Pages (from-to)1494-1498
Number of pages5
JournalPlanta Medica
Volume75
Issue number14
DOIs
Publication statusPublished - 2009 Dec 1

Keywords

  • Artekeiskeanol A
  • Artemisia keiskeana Miq.
  • Compositae
  • Degranulation
  • IL-13
  • RBL-2H3 cells
  • TNF-α

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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