TY - JOUR
T1 - Suppression of osteoclastic activities by calcitonin in the scales of goldfish (freshwater teleost) and nibbler fish (seawater teleost)
AU - Suzuki, Nobuo
AU - Suzuki, Tohru
AU - Kurokawa, Tadahide
N1 - Funding Information:
This study was supported in part by a grant to T.S. (Development of fundamental techniques for effective genetic improvement of aquatic organisms) sponsored by the Ministry of Agriculture, Forestry and Fisheries of Japan.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/1
Y1 - 2000/1
N2 - Using a tartrate-resistant acid phosphatase (TRACP) activity, the effects of calcitonin (CT) and estradiol-17β(E2) on osteoclastic activities in cultured scales of goldfish (freshwater fish) and nibbler fish (seawater fish) were examined. In mature male and female goldfish, scales were collected and incubated in Earle's minimun essential medium (MEM) supplemented with either CT (100 ng/ml) or E2 (100 ng/ml). In both sexes, CT suppressed TRACP activities in the scales, whereas E2 increased them. When CT (100 ng/ml) and E2 (100 ng/ml) were added simultaneously to MEM, TRACP activities in the scales did not change as compared with the control. Similar results were obtained with the scales of female nibbler fish. In goldfish, furthermore, the activation of scale TRACP by E2 was suppressed, depending on CT concentrations (100 pg/ml to 1 μg/ml). In fish reproduction, this function of CT may be useful to protect scales from excess degradation of calcium at vitellogenesis. (C) 2000 Elsevier Science Inc.
AB - Using a tartrate-resistant acid phosphatase (TRACP) activity, the effects of calcitonin (CT) and estradiol-17β(E2) on osteoclastic activities in cultured scales of goldfish (freshwater fish) and nibbler fish (seawater fish) were examined. In mature male and female goldfish, scales were collected and incubated in Earle's minimun essential medium (MEM) supplemented with either CT (100 ng/ml) or E2 (100 ng/ml). In both sexes, CT suppressed TRACP activities in the scales, whereas E2 increased them. When CT (100 ng/ml) and E2 (100 ng/ml) were added simultaneously to MEM, TRACP activities in the scales did not change as compared with the control. Similar results were obtained with the scales of female nibbler fish. In goldfish, furthermore, the activation of scale TRACP by E2 was suppressed, depending on CT concentrations (100 pg/ml to 1 μg/ml). In fish reproduction, this function of CT may be useful to protect scales from excess degradation of calcium at vitellogenesis. (C) 2000 Elsevier Science Inc.
KW - Calcitonin
KW - Estradiol-17β
KW - Osteoclast
KW - Scale
KW - Tartrate-resistant acid phosphatase
KW - Teleost
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U2 - 10.1016/S0196-9781(99)00181-3
DO - 10.1016/S0196-9781(99)00181-3
M3 - Article
C2 - 10704727
AN - SCOPUS:0033994210
VL - 21
SP - 115
EP - 124
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 1
ER -