Suppression of laminin binding protein (37LBP/p40) expression affects angiogenesis in the murine lung tumor

M. Tanaka, K. Satoh, K. Tanaka, M. Abe, M. Isemura, Katsuyoshi Hori, Y. Sato, T. Nukiwa

Research output: Contribution to journalArticle

Abstract

Expression of the 37-kDa laminin binding protein (37LBP/p40), a precursor of the 67-kDa laminin receptor, is well correlated with biological aggressiveness of cancer cells. Details of the role of 37LBP/p40 are, however, not fully understood. To elucidate the role played by the 37LBP/p40 in cancer cells directly, a murine lung cancer cell line T11, the 37LBP/p40 expression of which was remarkably decreased, was established by introduction of the antisense 37LBP/p40 RNA by using a retroviral vector. As a result, the population doubling time of T11 was prolonged (60 h), compared with those of P29, non-transfected cell line (42 h) and TN2, a transfectant with vehicle only (40 h). When 5 x 10 5 cells were subcutaneously injected into syngenic mice, the mean survival time of the T11 recipient (77.0±14.8 days) was also significantly prolonged compared with those of P29 (34.8±5.5 days) and TN2 (36.7±6.1 days), (p<0.001). Immunohistochemistry demonstrated that the density of microvessels in the T11 tumor was lower than those in P29 and TN2 tumor (p<0.05). It was also shown that conditioned medium of T11 cells induced capillary endothelial cell migration less than those of P29 and T42 sense RNA-transfectant did (p<0.05). These results suggest that decreased expression of 37LBP/p40 may relate to low tumorigenicity and that this may be partly caused by affected neovascularization of the murine lung cancer.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalConnective Tissue
Volume28
Issue number4
Publication statusPublished - 1996 Dec 1

Keywords

  • angiogenesis
  • antisense RNA
  • laminin receptor
  • lung cancer
  • tumorigenicity

ASJC Scopus subject areas

  • Rheumatology

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    Tanaka, M., Satoh, K., Tanaka, K., Abe, M., Isemura, M., Hori, K., Sato, Y., & Nukiwa, T. (1996). Suppression of laminin binding protein (37LBP/p40) expression affects angiogenesis in the murine lung tumor. Connective Tissue, 28(4), 237-245.