Suppression of intracellular calcium levels and inhibition of degranulation in RBL-2H3 mast cells by the sesquiterpene lactone parthenolide

Jangja Hong, Suzue Aoyama, Noriyasu Hirasawa, Okpyo Zee, Kenji Ishihara, Chika Hashida, Michio Kimura, Toshio Seyama, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Pretreatment with parthenolide for 60 min inhibited the antigen-induced degranulation of RBL-2H3 mast cells; the IC50 value being 4.5 ± 0.4 μM. The inhibition was not due to suppression of the phosphatidylinositol 3-kinase pathway because the antigen-induced phosphorylation of Akt was not inhibited by parthenolide. The antigen-induced increase in intracellular calcium levels was prevented by parthenolide, suggesting that parthenolide inhibited the antigen-induced degranulation by suppressing an increase in intracellular calcium levels. In support of this, parthenolide was found to prevent ionomycin-induced degranulation by inhibiting an increase in intracellular calcium levels. Therefore, parthenolide inhibits the degranulation of mast cells by preventing an increase in intracellular calcium levels.

Original languageEnglish
Pages (from-to)252-256
Number of pages5
JournalPlanta Medica
Volume77
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Asteraceae
  • RBL2H3 mast cells
  • Tanacetum parthenium (L.) Sch. Bip.
  • degranulation
  • intracellular calcium
  • parthenolide
  • β-hexosaminidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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