Suppression of gene expression and production of interleukin 13 by dexamethasone in human peripheral blood mononuclear cells

Toshiaki Fushimi, Sanae Shimura, Satsuki Suzuki, Hiroki Saitoh, Hiroshi Okayama, Kunio Shirato

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We examined the effect of dexamethasone on the gene expression and production of interleukin (IL)-13 by human peripheral blood mononuclear cells from healthy controls. The gene expression was assessed semiquantitatively by sequential transcription polymerase chain reaction and Southern blot analysis, and the production of this cytokine was assessed by enzyme-linked immunosorbent assay (ELISA). Dexamethasone suppressed IL-13 gene expression induced by stimulation with phytohemagglutinin and phorbol 12-myristate 13- acetate in a dose-dependent manner, with 96% suppression at 10-6 M, and also suppressed the increased production of IL-13. This is suggested to be one of the mechanisms by which glucocorticoids suppress allergic inflammation.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalTohoku Journal of Experimental Medicine
Volume185
Issue number2
DOIs
Publication statusPublished - 1998 Jun

Keywords

  • Dexamethasone
  • Human mononuclear cells
  • Interleukin 13
  • Messenger RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Suppression of gene expression and production of interleukin 13 by dexamethasone in human peripheral blood mononuclear cells'. Together they form a unique fingerprint.

  • Cite this