Suppression of fibroblast cell growth by overexpression of LIM-kinase 1

Osamu Higuchi, Gyeong Hun Baeg, Tetsu Akiyama, Kensaku Mizuno

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

LIM-kinase 1 (LIMK1) is a serine/threonine kinase containing two LIM motifs at the N-terminus. The functional role of LIMK1 has remained unknown. In this study, we examined the role of LIMK1 in cell growth of fibroblasts. Induced expression of LIMK1 in NIH3T3 cells led to growth retardation. Transfection of LIMK1 sense cDNA into NIH3T3 and H-ras-transformed FYJ10 fibroblasts significantly suppressed colony formation of these cells. In contrast, transfection with LIMK1 antisense cDNA strongly stimulated colony formation of the NIH3T3 cells. These findings suggest that LIMK1 functions as a negative regulator of fibroblast cell growth, and may play a role in tumor suppression.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalFEBS Letters
Volume396
Issue number1
DOIs
Publication statusPublished - 1996 Oct 28
Externally publishedYes

Keywords

  • DHR/PDZ domain
  • Growth suppression
  • LIM motif
  • LIMK
  • Protein kinase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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