TY - JOUR
T1 - Suppression of conditioned odor approach by feeding is independent of taste and nutritional value in drosophila
AU - Gruber, Franz
AU - Knapek, Stephan
AU - Fujita, Michiko
AU - Matsuo, Koichiro
AU - Bräcker, Lasse
AU - Shinzato, Nao
AU - Siwanowicz, Igor
AU - Tanimura, Teiichi
AU - Tanimoto, Hiromu
N1 - Funding Information:
We thank A. Gruschka, T. Inoshita, and B. Mühlbauer for excellent technical assistance; P.A. Garrity and J.H. Park for fly stocks; and F. Marion-Poll for his advice on electrophysiology setup. We are also grateful to M. Heisenberg, L. Nevin, C. Wegener, G. Wegener, and A. Yarali for discussion and/or critical reading of the manuscript. This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to T.T.), Emmy-Noether Program from Deutsche Forschungsgemeinschaft (to H.T.), Bernstein Focus Learning from Bundesministerium für Bildung und Forschung (to H.T.), and Max-Planck-Gesellschaft (to H.T.).
PY - 2013/3/18
Y1 - 2013/3/18
N2 - Motivation controls behavior [1]. A variety of food-related behaviors undergo motivational modulation by hunger, satiety, and other states [2-4]. Here we searched for critical satiation factors modulating approach to an odor associated with sugar reward in Drosophila melanogaster. We selectively manipulated different parameters associated with feeding, such as internal glucose levels, and determined which are required for suppressing conditioned odor approach. Surprisingly, glucose levels in the hemolymph, nutritional value, sweetness of the food, and ingested volume (above a minimal threshold) did not influence behavior suppression. Instead, we found that the total osmolarity of ingested food is a critical satiation factor. In parallel, we found that conditioned approach is transiently suppressed by artificial stimulation of adipokinetic hormone (AKH) expressing corpora cardiaca cells, which causes elevation of hemolymph carbohydrate and lipid concentrations [5, 6]. This result implies that a rise in hemolymph osmolarity, without the experience of feeding, is sufficient to satiate conditioned odor approach. AKH stimulation did not affect innate sugar preference, suggesting that multiple satiation signals control different sets of appetitive behaviors.
AB - Motivation controls behavior [1]. A variety of food-related behaviors undergo motivational modulation by hunger, satiety, and other states [2-4]. Here we searched for critical satiation factors modulating approach to an odor associated with sugar reward in Drosophila melanogaster. We selectively manipulated different parameters associated with feeding, such as internal glucose levels, and determined which are required for suppressing conditioned odor approach. Surprisingly, glucose levels in the hemolymph, nutritional value, sweetness of the food, and ingested volume (above a minimal threshold) did not influence behavior suppression. Instead, we found that the total osmolarity of ingested food is a critical satiation factor. In parallel, we found that conditioned approach is transiently suppressed by artificial stimulation of adipokinetic hormone (AKH) expressing corpora cardiaca cells, which causes elevation of hemolymph carbohydrate and lipid concentrations [5, 6]. This result implies that a rise in hemolymph osmolarity, without the experience of feeding, is sufficient to satiate conditioned odor approach. AKH stimulation did not affect innate sugar preference, suggesting that multiple satiation signals control different sets of appetitive behaviors.
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U2 - 10.1016/j.cub.2013.02.010
DO - 10.1016/j.cub.2013.02.010
M3 - Article
C2 - 23477724
AN - SCOPUS:84875247275
VL - 23
SP - 507
EP - 514
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 6
ER -