Abstract
Clindamycin reduced not only the β-lactamase activity but also the MIC of ceftazidime in Enterobacter cloacae NUH10. In the presence of clindamycin, intracellular and extracellular the β-lactamase activities were only 10% and 50%, respectively, compared with the control. By immunocytochemical examinations, β-lactamase were located in the cytoplasmic space in the presence of clindamycin, whereas it was found in the periplasmic space when clindamycin was not present. Western blot analysis showed when a smaller quantity of proteins on the low-molecular side after treatment clindamycin. These findings suggested that clindamycin does not suppress the synthesis of β-lactamase in E. cloacae NUH10, but suppresses the secretion of cephalosporinase from the cytoplasmic space (precursor form) to the periplasmic space (mature form).
Original language | English |
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Pages (from-to) | 207-214 |
Number of pages | 8 |
Journal | Therapeutic Research |
Volume | 16 |
Issue number | 1 |
Publication status | Published - 1995 Jan 1 |
Keywords
- Enterobacter cloacae
- class C β-lactamase
- clindamycin
ASJC Scopus subject areas
- Medicine(all)