Suppression of class C β-lactamase in Enterobacter cloacae by clindamycin

Y. Ishii, Mitsuo Kaku, K. Yamaguchi

Research output: Contribution to journalArticlepeer-review

Abstract

Clindamycin reduced not only the β-lactamase activity but also the MIC of ceftazidime in Enterobacter cloacae NUH10. In the presence of clindamycin, intracellular and extracellular the β-lactamase activities were only 10% and 50%, respectively, compared with the control. By immunocytochemical examinations, β-lactamase were located in the cytoplasmic space in the presence of clindamycin, whereas it was found in the periplasmic space when clindamycin was not present. Western blot analysis showed when a smaller quantity of proteins on the low-molecular side after treatment clindamycin. These findings suggested that clindamycin does not suppress the synthesis of β-lactamase in E. cloacae NUH10, but suppresses the secretion of cephalosporinase from the cytoplasmic space (precursor form) to the periplasmic space (mature form).

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalTherapeutic Research
Volume16
Issue number1
Publication statusPublished - 1995 Jan 1

Keywords

  • Enterobacter cloacae
  • class C β-lactamase
  • clindamycin

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Suppression of class C β-lactamase in Enterobacter cloacae by clindamycin'. Together they form a unique fingerprint.

Cite this