Suppression by adrenoceptor beta-agonists of vascular permeability increase and edema formation induced by arachidonate metabolites, platelet-activating factor, and tumor-promoting phorbol ester TPA

Ohuchi Kazuo Ohuchi, Watanabe Masako Watanabe, Hirasawa Noriyasu Hirasawa, Yoshizaki Shiro Yoshizaki, Mue Suetsugu Mue, Tsurufuji Susumu Tsurufuji

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Air-pouch-type inflammation was induced by injecting sodium carboxymethyl cellulose solution containing leukotriene C4 (LTC4, 3.20 × 10-7 M, 0.2 μg/ml) and prostaglandin E2 (PGE2, 5.68 × 10-6 M, 2.0 μg/ml), platelet-activating factor (PAF, 1 × 10-6 M, 0.52 μg/ml), or 12-O-tetradecanoyl phorbol 13-acetate (TPA, 1.62 × 10-6 M, 1.0 μg/ml) into an air pouch made on the dorsum of rats. Vascular permeability and tissue edema formation were significantly increased by injecting the phlogogen solution. The histamine level in the pouch fluid was dramatically increased by injecting TPA but not by LTC4 and PGE2, or PAF. Injection of isoproterenol or procaterol with the phlogogen solution produced dose-dependent suppression of both vascular permeability increase and tissue edema formation. However, the TPA-induced increase in the histamine level was not suppressed in parallel with the decrease of vascular permeability or tissue edema formation. These results indicate that beta-agonists suppress vascular permeability response and local tissue edema formation not by inhibiting mast cell degranulation, but by inhibiting the reactivity of the local vasculature to chemical mediators such as arachidonate metabolites, PAF, and histamine and serotonin released from mast cells.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalImmunopharmacology
Volume20
Issue number2
DOIs
Publication statusPublished - 1990 Jan 1

Keywords

  • Beta-agonist
  • Edema
  • Leukotriene C
  • Platelet-activating factor
  • Prostaglandin E
  • Tumor promoter
  • Vascular permeability

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Suppression by adrenoceptor beta-agonists of vascular permeability increase and edema formation induced by arachidonate metabolites, platelet-activating factor, and tumor-promoting phorbol ester TPA'. Together they form a unique fingerprint.

Cite this