Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

K. Katoh; A. Matsuda; A. Ishigami; S. Yonekura; H. Ishiwata; C. Chen; Y. Obara

doi:10.1016/j.cellbi.2004.03.016

Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

K. Katoh, A. Matsuda, A. Ishigami, S. Yonekura, H. Ishiwata, C. Chen, Y. Obara

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]_c) and calcium ion ([Ca²⁺]_c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10^-6 to 10 ^-3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10^-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10^-8M)-induced increases in [cAMP]_c and [Ca ²⁺]_c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.

Original language	English
Pages (from-to)	463-469
Number of pages	7
Journal	Cell Biology International
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/j.cellbi.2004.03.016
Publication status	Published - 2004 Jun

Keywords

Anterior pituitary cells
Bisphenol A
Cyclic AMP
Cytosolic calcium
GH release
GHmRNA
Sheep

ASJC Scopus subject areas

Cell Biology

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title = "Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells",

abstract = "We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.",

keywords = "Anterior pituitary cells, Bisphenol A, Cyclic AMP, Cytosolic calcium, GH release, GHmRNA, Sheep",

author = "K. Katoh and A. Matsuda and A. Ishigami and S. Yonekura and H. Ishiwata and C. Chen and Y. Obara",

note = "Funding Information: This manuscript includes a part of a Master Thesis by A. Matsuda submitted to Tohoku University, 2001. We gratefully acknowledge Dr. M.T. Rose (University of Wales, Aberystwyth, UK) for his kind advice on this manuscript and Dr. A.F. Parlow for the ovine GH and antibody provided by NIDDK, USA. This study was partly supported by the grant from National Institute of Animal Health (I-2-03), Japan.",

year = "2004",

month = jun,

doi = "10.1016/j.cellbi.2004.03.016",

language = "English",

volume = "28",

pages = "463--469",

journal = "Cell Biology International Reports",

issn = "1065-6995",

publisher = "Portland Press Ltd.",

number = "6",

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T1 - Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

AU - Katoh, K.

AU - Matsuda, A.

AU - Ishigami, A.

AU - Yonekura, S.

AU - Ishiwata, H.

AU - Chen, C.

AU - Obara, Y.

N1 - Funding Information: This manuscript includes a part of a Master Thesis by A. Matsuda submitted to Tohoku University, 2001. We gratefully acknowledge Dr. M.T. Rose (University of Wales, Aberystwyth, UK) for his kind advice on this manuscript and Dr. A.F. Parlow for the ovine GH and antibody provided by NIDDK, USA. This study was partly supported by the grant from National Institute of Animal Health (I-2-03), Japan.

PY - 2004/6

Y1 - 2004/6

N2 - We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.

AB - We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.

KW - Anterior pituitary cells

KW - Bisphenol A

KW - Cyclic AMP

KW - Cytosolic calcium

KW - GH release

KW - GHmRNA

KW - Sheep

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Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

Abstract

Keywords

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