Abstract
We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.
Original language | English |
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Pages (from-to) | 463-469 |
Number of pages | 7 |
Journal | Cell Biology International |
Volume | 28 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2004 Jun |
Keywords
- Anterior pituitary cells
- Bisphenol A
- Cyclic AMP
- Cytosolic calcium
- GH release
- GHmRNA
- Sheep
ASJC Scopus subject areas
- Cell Biology