Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

K. Katoh, A. Matsuda, A. Ishigami, S. Yonekura, H. Ishiwata, C. Chen, Y. Obara

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    10 Citations (Scopus)


    We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.

    Original languageEnglish
    Pages (from-to)463-469
    Number of pages7
    JournalCell Biology International
    Issue number6
    Publication statusPublished - 2004 Jun


    • Anterior pituitary cells
    • Bisphenol A
    • Cyclic AMP
    • Cytosolic calcium
    • GH release
    • GHmRNA
    • Sheep

    ASJC Scopus subject areas

    • Cell Biology


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