TY - JOUR
T1 - Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells
AU - Katoh, K.
AU - Matsuda, A.
AU - Ishigami, A.
AU - Yonekura, S.
AU - Ishiwata, H.
AU - Chen, C.
AU - Obara, Y.
PY - 2004/6/1
Y1 - 2004/6/1
N2 - We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.
AB - We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10-6 to 10 -3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10-4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10-8M)-induced increases in [cAMP]c and [Ca 2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.
KW - Anterior pituitary cells
KW - Bisphenol A
KW - Cyclic AMP
KW - Cytosolic calcium
KW - GH release
KW - GHmRNA
KW - Sheep
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U2 - 10.1016/j.cellbi.2004.03.016
DO - 10.1016/j.cellbi.2004.03.016
M3 - Article
C2 - 15223023
AN - SCOPUS:3042633473
VL - 28
SP - 463
EP - 469
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 6
ER -