Superoxide anion generation by pacific oyster (Crassostrea gigas) hemocytes: Identification by electron spin resonance spin trapping and chemiluminescence analysis

Keisuke Takahashi, Takaaki Akaike, Keizo Sato, Katsuyoshi Mori, Hiroshi Maeda

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

1. 1. The generation of a superoxide anion (O2•-) by hemocytes of the Pacific oyster, Crassostrea gigas, was investigated by using electron spin resonance spin trapping with the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and by the chemiluminescence method. 2. 2. Oyster hemocytes released O2•- on stimulation with phorbol myristate acetate as revealed by both methods. 3. 3. A great difference was found in O2•--generating activity among three subpopulations of hemocytes, which were separated from each other by using Percoll density gradients. 4. 4. Evidence obtained in our experiments suggests that a specific O2•--forming system, which becomes functional upon adequate stimulation, exists in a specialized subpopulation of C. gigas hemocytes similar to the professional phagocytes in mammals.

Original languageEnglish
Pages (from-to)32-41
Number of pages10
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume105
Issue number1
DOIs
Publication statusPublished - 1993 May

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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