33S nuclear magnetic resonance spectroscopy of biological samples obtained with a laboratory model 33S cryogenic probe

Fumio Hobo, Masato Takahashi, Yuta Saito, Naoki Sato, Tomoaki Takao, Seizo Koshiba, Hideaki Maeda

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

33S nuclear magnetic resonance (NMR) spectroscopy is limited by inherently low NMR sensitivity because of the quadrupolar moment and low gyromagnetic ratio of the S 33 nucleus. We have developed a 10 mm S 33 cryogenic NMR probe, which is operated at 9-26 K with a cold preamplifier and a cold rf switch operated at 60 K. The S 33 NMR sensitivity of the cryogenic probe is as large as 9.8 times that of a conventional 5 mm broadband NMR probe. The S 33 cryogenic probe was applied to biological samples such as human urine, bile, chondroitin sulfate, and scallop tissue. We demonstrated that the system can detect and determine sulfur compounds having SO 4 2- anions and - SO 3- groups using the S 33 cryogenic probe, as the S 33 nuclei in these groups are in highly symmetric environments. The NMR signals for other common sulfur compounds such as cysteine are still undetectable by the S 33 cryogenic probe, as the S 33 nuclei in these compounds are in asymmetric environments. If we shorten the rf pulse width or decrease the rf coil diameter, we should be able to detect the NMR signals for these compounds.

Original languageEnglish
Article number054302
JournalReview of Scientific Instruments
Volume81
Issue number5
DOIs
Publication statusPublished - 2010 May 1
Externally publishedYes

ASJC Scopus subject areas

  • Instrumentation

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