1H NMR spectrsoscopic quantification of plasma metabolites in dialysate during hemodialysis

Itiro Ando, Kazuhisa Takeuchi, Shiro Oguma, Hiroshi Sato, Hiroshi Sekino, Yutaka Imai, And Masako Fujiwara

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We used 1H nuclear magnetic resonance (NMR) spectroscopy to assess metabolic responses in patients undergoing hemodialysis (HD). We collected 71 samples of plasma and dialysate from 10 patients before, during, and after HD. We used the dialysate as a possible substitute for blood plasma to quantify small metabolites by 1H NMR. We con- firmed TSP (sodium 3-(trimethylsilyl) propionate 2, 2, 3, 3-d4) as a reference of NMR intensity in dialysate. We examined TSP sensitivities in various dialysate spectra and the correlation between signal intensities and added quantities of TSP. We used integrations of signal areas on 1H NMR spectra of plasma and dialysate to quantify concentrations of creatinine, lactate, alanine, and valine and calculate their ratios between plasma and dialysate. The ratios of metabolites in plasma to dialysate were 3.2±0.4 (creatinine), 3.6±0.5 (valine), 3.8±0.7 (alanine), and 4.0±0.8 (lactate) mM (mean±standard deviation [SD]). The broader distributions of ratios in levels of lactate and alanine suggested their de novo production during the HD session. Estimation of blood metabolite levels using dialysate is useful for quantitative analysis of metabolic status in blood during HD.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalMagnetic Resonance in Medical Sciences
Volume12
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Alanine
  • Creatinine
  • Lactate
  • Metabolomics
  • Valine

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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