19F MRI Monitoring of Gene Expression in Living Cells through Cell-Surface β-Lactamase Activity

Hisashi Matsushita, Shin Mizukami, Yuki Mori, Fuminori Sugihara, Masashiro Shirakawa, Yoshichika Yoshioka, Kazuya Kikuchi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Magnetic resonance imaging provides important intravital information on deep tissues that cannot be visualized by other methods. Although we had previously developed an off/on switching 19F MRI probe to monitor reporter enzyme activity on the basis of the paramagnetic relaxation enhancement effect, it was difficult to monitor biological events in living cells because the 19F MRI probe did not permeate living cell membrane. In this study, we have developed a new 19F MRI system for monitoring gene expression in living cells by exploiting cell-surface-displayed β-lactamase and the specifically designed 19F MRI probe. By using this system, cellular gene expression was successfully detected by 19F MRI without cell fixation. This imaging strategy shows promise for monitoring in vivo gene expression, and therefore it could lead to useful technologies for the diagnosis and therapy of various diseases.

Original languageEnglish
Pages (from-to)1579-1583
Number of pages5
JournalChemBioChem
Volume13
Issue number11
DOIs
Publication statusPublished - 2012 Jul 23

Keywords

  • Beta-lactamase
  • F MRI
  • Gene expression
  • Membrane proteins
  • Paramagnetic resonance enhancement
  • Self-elimination properties

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Matsushita, H., Mizukami, S., Mori, Y., Sugihara, F., Shirakawa, M., Yoshioka, Y., & Kikuchi, K. (2012). 19F MRI Monitoring of Gene Expression in Living Cells through Cell-Surface β-Lactamase Activity. ChemBioChem, 13(11), 1579-1583. https://doi.org/10.1002/cbic.201200331