[11C] Methylation on a C18 Sep-Pak cartridge: A convenient way to produce [N-methyl-11C] choline

Claudio Pascali, Anna Bogni, Ren Iwata, Mara Cambiè, Emilio Bombardieri

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    96 Citations (Scopus)


    [N-Methyl-11C]choline has been simply and efficiently labelled at room temperature by solid supported [11C]methylation of 2-dimethylaminoethanol on a commercial C18 Sep-Pak Light cartridge. The labelled product was retained on a cation exchange resin (Sep-Pak Plus Accell Plus CM) and washed with ethanol and water to remove any excess precursor. Then [N-methyl-11C]choline was desorbed from the cartridge by elution with saline, passed through a 0.2 mm sterile filter and collected in a sterile vial. Overall, the total synthesis time was 12 min from the end of bombardment (EOB). The radiochemical yield from [11C]CO2, decay corrected (EOB) was 87 and the radiochemical purity was greater than 99. Very conveniently, the automated apparatus employed is a very simple modification of the system routinely used for the synthesis of L-[S-methyl-11C]methionine.

    Original languageEnglish
    Pages (from-to)195-203
    Number of pages9
    JournalJournal of Labelled Compounds and Radiopharmaceuticals
    Issue number2
    Publication statusPublished - 2000 Jan 1


    • PET
    • Solid supported methylation
    • [C] choline

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Radiology Nuclear Medicine and imaging
    • Drug Discovery
    • Spectroscopy
    • Organic Chemistry


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