Sulfur Isotopic Fractionation of Carbonyl Sulfide during Degradation by Soil Bacteria

Kazuki Kamezaki, Shohei Hattori, Takahiro Ogawa, Sakae Toyoda, Hiromi Kato, Yoko Katayama, Naohiro Yoshida

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We performed laboratory incubation experiments on the degradation of gaseous phase carbonyl sulfide (OCS) by soil bacteria to determine its sulfur isotopic fractionation constants (34ϵ). Incubation experiments were conducted using strains belonging to the genera Mycobacterium, Williamsia, and Cupriavidus isolated from natural soil environments. The 34ϵ values determined were -3.67 ± 0.33‰, -3.99 ± 0.19‰, -3.57 ± 0.22‰, and -3.56 ± 0.23‰ for Mycobacterium spp. strains THI401, THI402, THI404, and THI405; -3.74 ± 0.29‰ for Williamsia sp. strain THI410; and -2.09 ± 0.07‰ and -2.38 ± 0.35‰ for Cupriavidus spp. strains THI414 and THI415. Although OCS degradation rates divided by cell numbers (cell-specific activity) were different among strains of the same genus, the 34ϵ values for same genus showed no significant differences. Even though the numbers of bacterial species examined were limited, our results suggest that 34ϵ values for OCS bacterial degradation depend not on cell-specific activities, but on genus-level biological differences, suggesting that 34ϵ values are dependent on enzymatic and/or membrane properties. Taking our 34ϵ values as representative for bacterial OCS degradation, the expected atmospheric changes in δ34S values of OCS range from 0.5‰ to 0.9‰, based on previously reported decreases in OCS concentrations at Mt. Fuji, Japan. Consequently, tropospheric observation of δ34S values for OCS coupled with 34ϵ values for OCS bacterial degradation can potentially be used to investigate soil as an OCS sink. (Chemical Equation Presented).

Original languageEnglish
Pages (from-to)3537-3544
Number of pages8
JournalEnvironmental Science and Technology
Volume50
Issue number7
DOIs
Publication statusPublished - 2016 Apr 5

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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