Abstract
Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.
Original language | English |
---|---|
Number of pages | 1 |
Publication status | Published - 2005 Dec 1 |
Event | 54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sep 20 → 2005 Sep 22 |
Other
Other | 54th SPSJ Symposium on Macromolecules |
---|---|
Country/Territory | Japan |
City | Yamagata |
Period | 05/9/20 → 05/9/22 |
Keywords
- Enzymatic polymer reaction
- Polysaccharide
- Sulfatase
- Sulfation
ASJC Scopus subject areas
- Engineering(all)