Sulfate-transferring to carbohydrates using enzymatic polymer reaction

Tomohiko Kotake, Hironori Izawa, Masato Noguchi, Atsushi Kobayashi, Shin Ichiro Shoda

Research output: Contribution to conferencePaperpeer-review

Abstract

Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Enzymatic polymer reaction
  • Polysaccharide
  • Sulfatase
  • Sulfation

ASJC Scopus subject areas

  • Engineering(all)

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