Sulfate-transferring to carbohydrates using enzymatic polymer reaction

Tomohiko Kotake; Hironori Izawa; Masato Noguchi; Atsushi Kobayashi; Shin Ichiro Shoda

Sulfate-transferring to carbohydrates using enzymatic polymer reaction

Tomohiko Kotake, Hironori Izawa, Masato Noguchi, Atsushi Kobayashi, Shin Ichiro Shoda

ENG - Biomolecular Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sep 20 → 2005 Sep 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

Enzymatic polymer reaction
Polysaccharide
Sulfatase
Sulfation

ASJC Scopus subject areas

Engineering(all)

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title = "Sulfate-transferring to carbohydrates using enzymatic polymer reaction",

abstract = "Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.",

keywords = "Enzymatic polymer reaction, Polysaccharide, Sulfatase, Sulfation",

author = "Tomohiko Kotake and Hironori Izawa and Masato Noguchi and Atsushi Kobayashi and Shoda, {Shin Ichiro}",

year = "2005",

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note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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TY - CONF

T1 - Sulfate-transferring to carbohydrates using enzymatic polymer reaction

AU - Kotake, Tomohiko

AU - Izawa, Hironori

AU - Noguchi, Masato

AU - Kobayashi, Atsushi

AU - Shoda, Shin Ichiro

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.

AB - Sulfated carbohydrates play an important role in biological phenomena. The conventional chemical methods for sulfation of oligosaccharides involve tedious procedures. In this study, we developed a novel method for the synthesis of sulfated carbohydrates based on enzymatic polymer reaction. To obtain enzymes that show sulfate-transferring activity onto a polysaccharide, arylsulfatases with hydrolytic activity towards arylsulfate derivative was screened. A crude enzyme powders from Aspergillus oryzae showed arylsulafatase activity. Sulfatase-catalyzed sulfation was demonstrated by using p-nitrophenyl sulfate as a sulfate donor and chitooligosaccharides as acceptor, giving rise to a mixture of partially sulfated chitooligosaccharides.

KW - Enzymatic polymer reaction

KW - Polysaccharide

KW - Sulfatase

KW - Sulfation

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UR - http://www.scopus.com/inward/citedby.url?scp=33645637729&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645637729

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Sulfate-transferring to carbohydrates using enzymatic polymer reaction

Abstract

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Keywords

ASJC Scopus subject areas

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