Sugar-sensitive thin films composed of concanavalin A and sugar-bearing polymers

Katsuhiko Sato, Daisuke Kodama, Jun Ichi Anzai

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Layered thin films composed of concanavalin A (Con A) and sugar-bearing polymers were prepared by a layer-by-layer deposition of Con A and the polymer on a solid surface. The sugar-induced disintegration was studied. Con A-polymer layered films could be successfully prepared using a maltose-bearing polymer (PV-MA), while melibiose- and glucose-bearing polymers (PV-MEA and PV-G) did not afford a layered film, due to a weak affinity of PV-MEA and PV-G to Con A. The Con A/PV-MA layered film was stable in pH 7 and 8 solutions, while in a pH 6 medium the film was slightly unstable. The Con A/PV-MA film was disintegrated upon the addition of sugars in solution owing to a preferential binding of the sugars to the binding site of Con A in the film. The disintegration rate was dependent on the type of sugar and its concentration. The Con A/PV-MA film was disintegrated rapidly upon the addition of methyl α-D-mannopyranoside, while the rate was slower upon the addition of the same concentration of D-mannose, D-glucose and methyl α-D-glucopyranoside. The present system may be useful for constructing sensitive devices that can release a drug or other functional molecules in response to sugars. 2005

Original languageEnglish
Pages (from-to)1375-1378
Number of pages4
Journalanalytical sciences
Volume21
Issue number11
DOIs
Publication statusPublished - 2005 Nov

ASJC Scopus subject areas

  • Analytical Chemistry

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