Sugar-inducible expression of the nucleolin-1 gene of Arabidopsis thaliana and its role in ribosome synthesis, growth and development

Hisae Kojima, Takamasa Suzuki, Takenori Kato, Ken Ichi Enomoto, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Julio Sáez-Vasquez, Manuel Echeverría, Tsuyoshi Nakagawa, Sumie Ishiguro, Kenzo Nakamura

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Animal and yeast nucleolin function as global regulators of ribosome synthesis, and their expression is tightly linked to cell proliferation. Although Arabidopsis contains two genes for nucleolin, AtNuc-L1 is the predominant if not only form of the protein found in most tissues, and GFP-AtNuc-L1 fusion proteins were targeted to the nucleolus. Expression of AtNuc-L1 was strongly induced by sucrose or glucose but not by non-metabolizable mannitol or 2-deoxyglucose. Sucrose also caused enhanced expression of genes for subunits of C/D and H/ACA small nucleolar ribonucleoproteins, as well as a large number of genes for ribosomal proteins (RPs), suggesting that carbohydrate availability regulates de novo ribosome synthesis. In sugar-starved cells, induction of AtNuc-L1 occurred with 10 mm glucose, which seemed to be a prerequisite for resumption of growth. Disruption of AtNuc-L1 caused an increased steady-state level of pre-rRNA relative to mature 25S rRNA, and resulted in various phenotypes that overlap those reported for several RP gene mutants, including a reduced growth rate, prolonged lifetime, bushy growth, pointed leaf, and defective vascular patterns and pod development. These results suggest that the rate of ribosome synthesis in the meristem has a strong impact not only on the growth but also the structure of plants. The AtNuc-L1 disruptant exhibited significantly reduced sugar-induced expression of RP genes, suggesting that AtNuc-L1 is involved in the sugar-inducible expression of RP genes.

Original languageEnglish
Pages (from-to)1053-1063
Number of pages11
JournalPlant Journal
Volume49
Issue number6
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • Nucleolin
  • Ribosomal protein genes
  • Ribosome synthesis
  • Sugar
  • snoRNP

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Sugar-inducible expression of the nucleolin-1 gene of Arabidopsis thaliana and its role in ribosome synthesis, growth and development'. Together they form a unique fingerprint.

  • Cite this

    Kojima, H., Suzuki, T., Kato, T., Enomoto, K. I., Sato, S., Kato, T., Tabata, S., Sáez-Vasquez, J., Echeverría, M., Nakagawa, T., Ishiguro, S., & Nakamura, K. (2007). Sugar-inducible expression of the nucleolin-1 gene of Arabidopsis thaliana and its role in ribosome synthesis, growth and development. Plant Journal, 49(6), 1053-1063. https://doi.org/10.1111/j.1365-313X.2006.03016.x