Sucrose and cytokinin modulation of WPK4, a gene encoding a SNF1-related protein kinase from wheat

Yoshihisa Ikeda, Nozomu Koizumi, Tomonobu Kusano, Hiroshi Sano

    Research output: Contribution to journalArticlepeer-review

    50 Citations (Scopus)

    Abstract

    WPK4, a gene encoding a putative protein kinase, was initially identified in wheat (Triticum aestivum) and shown to be upregulated by light, nutrient deprivation, and cytokinins. To confirm that WPK4 has protein kinase activity, the protein was produced in Escherichia coli as a fusion protein with glutathione S-transferase. The purified protein exhibited autophosphorylation activity and phosphorylated both myelin basic protein and a peptide fragment of rice 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Levels of WPK4 transcripts in wheat seedlings were increased and decreased by the removal and addition of sucrose (Suc), respectively, to the culture medium. The introduction of the N-terminal kinase region of WPK4 into the yeast snf1 mutant cells, which cannot utilize Suc as a carbon source, rescued growth in Suc-containing medium. Cytokinins up-regulated the accumulation of WPK4 transcripts, but their effects were cancelled by the addition of Suc. Our results suggest that Suc negatively regulates the signaling pathway in which transcriptional activation of WPK4 is mediated by cytokinins.

    Original languageEnglish
    Pages (from-to)813-820
    Number of pages8
    JournalPlant physiology
    Volume121
    Issue number3
    DOIs
    Publication statusPublished - 1999 Nov

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science

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