Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture

Takeshi Akao, Katsuya Gomi, Kuniyasu Goto, Naoto Okazaki, Osamu Akita

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)

    Abstract

    In solid-state cultures (SC), Aspergillus oryzae shows characteristics such as high-level production and secretion of enzymes and hyphal differentiation with asexual development which are absent in liquid (submerged) culture (LC). It was predicted that many of the genes involved in the characteristics of A. oryzae in SC are differentially expressed between SC and LC. We generated two subtracted cDNA libraries with bi-directional cDNA subtractive hybridizations to isolate and identify such genes. Among them, we identified genes upregulated in or specific to SC, such as the AOS (A. oryzae SC-specific gene) series, and those downregulated or not expressed in SC, such as the AOL (A. oryzae LC-specific) series. Sequencing analyses revealed that the AOS series and the AOL series contain genes encoding extra- and intracellular enzymes and transport proteins. However, half were functionally unclassified by nucleotide sequences. Also, by expression profile, the AOS series comprised two groups. These gene products' molecular functions and physiological roles in SC await further investigation.

    Original languageEnglish
    Pages (from-to)275-281
    Number of pages7
    JournalCurrent Genetics
    Volume41
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Aspergillus oryzae
    • Differential gene expression
    • Solid-state culture
    • cDNA subtraction

    ASJC Scopus subject areas

    • Genetics

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