Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture

Takeshi Akao, Katsuya Gomi, Kuniyasu Goto, Naoto Okazaki, Osamu Akita

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In solid-state cultures (SC), Aspergillus oryzae shows characteristics such as high-level production and secretion of enzymes and hyphal differentiation with asexual development which are absent in liquid (submerged) culture (LC). It was predicted that many of the genes involved in the characteristics of A. oryzae in SC are differentially expressed between SC and LC. We generated two subtracted cDNA libraries with bi-directional cDNA subtractive hybridizations to isolate and identify such genes. Among them, we identified genes upregulated in or specific to SC, such as the AOS (A. oryzae SC-specific gene) series, and those downregulated or not expressed in SC, such as the AOL (A. oryzae LC-specific) series. Sequencing analyses revealed that the AOS series and the AOL series contain genes encoding extra- and intracellular enzymes and transport proteins. However, half were functionally unclassified by nucleotide sequences. Also, by expression profile, the AOS series comprised two groups. These gene products' molecular functions and physiological roles in SC await further investigation.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalCurrent Genetics
Volume41
Issue number4
DOIs
Publication statusPublished - 2002 Dec 1

Keywords

  • Aspergillus oryzae
  • Differential gene expression
  • Solid-state culture
  • cDNA subtraction

ASJC Scopus subject areas

  • Genetics

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