Subnanoliter volume wall-jet cells combined with interdigitated microarray electrode and enzyme modified planar microelectrode

Osamu Niwa, Ryoji Kurita, Zhiming Liu, Tsutomu Horiuchi, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Miniaturized wall-jet type flow cells with an active volume of 0.042-15 nL were fabricated for use as highly sensitive electrochemical detectors for capillary electrophoresis/electrochemical detection and small on-line enzyme sensors. The cells consisted of three glass plates and a fused-silica capillary. Two of the plates had microfabricated flow channels and guide trenches for the capillary and working, reference, and counter electrodes. The other plate had a film electrode. When an interdigitated microarray electrode (total area, 66 μm x 64 μm; bandwidth and gap, 2 μm) was installed in the flow cell, the redox cycling enhanced the current at flow rates of less than 100 nL/min even though there were only eight pairs of microbands. A sharp dopamine peak enhanced by the redox cycling was observed when the cell was used for capillary electrophoresis. A square film electrode modified with glutamate oxidase and Os-poly(vinylpyridine) containing HRP was also installed in the flow cell and used to measure neurotransmitter release from cultured nerve cells. When the flow rate was relatively high, the response time of the modified electrode was comparable to that of a cylindrical carbon fiber electrode (33 μm o.d.) modified with the same enzyme and mediator. We observed a transient cathodic current response assigned to the glutamate release with the electrode in the flow cell in a suction mode measurement when we stimulated cultured nerve cells electrically with a dual microelectrode.

Original languageEnglish
Pages (from-to)949-955
Number of pages7
JournalAnalytical Chemistry
Volume72
Issue number5
DOIs
Publication statusPublished - 2000 Mar 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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