TY - JOUR
T1 - Study of the acute cardiovascular effects of several antihypertensive agents with the measurement of plasma catecholamines in mice
AU - Tsunoda, Makoto
AU - Yamagishi, Masanori
AU - Imai, Kazuhiro
AU - Yanagisawa, Teruyuki
PY - 2009/6
Y1 - 2009/6
N2 - A highly sensitive determination method was established for catecholamines (norepinephrine (NE), epinephrine, and dopamine) with high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection. In this study, the method was applied to mouse plasma, and it was determined that only 10 μl of mouse plasma was necessary for the selective and reproducible determination of catecholamines. Studies were then conducted in acute cardiovascular effects of sodium nitroprusside, nicardipine, captopril (angiotensin-converting enzyme (ACE) inhibitor), candesartan, and olmesartan (type 1 angiotensin receptor antagonists (AT1 antagonists)) by this method. Sodium nitroprusside and nicardipine elevated plasma NE concentrations significantly, whereas the ACE inhibitor and the AT1 antagonists did not change plasma NE concentrations in anesthetized mice. These results suggested that angiotensin II-induced augmentation may be mainly carried through the central baroreflex pathway.
AB - A highly sensitive determination method was established for catecholamines (norepinephrine (NE), epinephrine, and dopamine) with high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection. In this study, the method was applied to mouse plasma, and it was determined that only 10 μl of mouse plasma was necessary for the selective and reproducible determination of catecholamines. Studies were then conducted in acute cardiovascular effects of sodium nitroprusside, nicardipine, captopril (angiotensin-converting enzyme (ACE) inhibitor), candesartan, and olmesartan (type 1 angiotensin receptor antagonists (AT1 antagonists)) by this method. Sodium nitroprusside and nicardipine elevated plasma NE concentrations significantly, whereas the ACE inhibitor and the AT1 antagonists did not change plasma NE concentrations in anesthetized mice. These results suggested that angiotensin II-induced augmentation may be mainly carried through the central baroreflex pathway.
KW - Angiotensin II
KW - Chemiluminescence
KW - HPLC
KW - Sympathetic nervous system
UR - http://www.scopus.com/inward/record.url?scp=67349200971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349200971&partnerID=8YFLogxK
U2 - 10.1007/s00216-009-2685-0
DO - 10.1007/s00216-009-2685-0
M3 - Article
C2 - 19234691
AN - SCOPUS:67349200971
VL - 394
SP - 947
EP - 952
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 1618-2642
IS - 4
ER -