Study of the acute cardiovascular effects of several antihypertensive agents with the measurement of plasma catecholamines in mice

Makoto Tsunoda, Masanori Yamagishi, Kazuhiro Imai, Teruyuki Yanagisawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A highly sensitive determination method was established for catecholamines (norepinephrine (NE), epinephrine, and dopamine) with high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection. In this study, the method was applied to mouse plasma, and it was determined that only 10 μl of mouse plasma was necessary for the selective and reproducible determination of catecholamines. Studies were then conducted in acute cardiovascular effects of sodium nitroprusside, nicardipine, captopril (angiotensin-converting enzyme (ACE) inhibitor), candesartan, and olmesartan (type 1 angiotensin receptor antagonists (AT1 antagonists)) by this method. Sodium nitroprusside and nicardipine elevated plasma NE concentrations significantly, whereas the ACE inhibitor and the AT1 antagonists did not change plasma NE concentrations in anesthetized mice. These results suggested that angiotensin II-induced augmentation may be mainly carried through the central baroreflex pathway.

Original languageEnglish
Pages (from-to)947-952
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume394
Issue number4
DOIs
Publication statusPublished - 2009 Jun

Keywords

  • Angiotensin II
  • Chemiluminescence
  • HPLC
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'Study of the acute cardiovascular effects of several antihypertensive agents with the measurement of plasma catecholamines in mice'. Together they form a unique fingerprint.

Cite this