Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope

Yoshiki Nakata, Yuichiro Shimizu, Takehiro Suzuki, Masaya Fujita, Kazue Kurihara

Research output: Contribution to conferencePaperpeer-review

Abstract

Transcription factor protein, Spo0A-DB, of Bacillus Subtilis recognize base sequence TGTCGAA called 0Abox. We measured interactions between Spo0A-DB and DNA at various Spo0A-DB densities using colloid probe atomic force microscope. For this interaction measurement, we found the optimum density of Spo0A-DB which corresponded to the closest packing. At excess adsorbed conditions, Spo0A-DB isn't in an appropriate orientation or the DNA binding site of SpoOA-DB is blocked by others, and at smaller densities, influences of the bottom chelate layer is increased.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Bacillus Subtilis
  • Colloid probe atomic force microscope
  • Interaction between protein and DNA
  • Spo0A-DB

ASJC Scopus subject areas

  • Engineering(all)

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