TY - JOUR
T1 - Study of drug effects of calcium channel blockers on retinal degeneration of rd mouse
AU - Takano, Yoshiko
AU - Ohguro, Hiroshi
AU - Dezawa, Mari
AU - Ishikawa, Hiroto
AU - Yamazaki, Hitoshi
AU - Ohguro, Ikuyo
AU - Mamiya, Kazuhisa
AU - Metoki, Tomomi
AU - Ishikawa, Futoshi
AU - Nakazawa, Mitsuru
PY - 2004/1/23
Y1 - 2004/1/23
N2 - In the present study, we studied drug effects of Ca2+ antagonists on the retinal degeneration of rd mouse to evaluate their efficacy. Several kinds of Ca2+ antagonists, diltiazem, nicardipine, nilvadipine or nifedipine were administrated intraperitoneally and thereafter retinal morphology and functions were analyzed. In addition, we performed DNA microarray analysis both in nilvadipine treated and control retinas to understand their drug effects at molecular levels. We found that nilvadipine caused significant preservation of retinal thickness in rd mouse during the initial stage of the retinal degeneration, and nicardipine showed also significant but lesser preservation than nilvadipine. However, we recognized no preservation effects of diltiazem and nifedipine. In the total 3774 genes, the expressions of 27 genes were altered upon administration of nilvadipine, including several genes related to the apoptotic pathway, neuro-survival factor, Ca2+ metabolisms, and other mechanisms. It is suggested that some types of Ca2+ channel blockers, such as nilvadipine and nicardipine, are able to preserve photoreceptor cells in rd mouse and can potentially be used to treat some RP patients.
AB - In the present study, we studied drug effects of Ca2+ antagonists on the retinal degeneration of rd mouse to evaluate their efficacy. Several kinds of Ca2+ antagonists, diltiazem, nicardipine, nilvadipine or nifedipine were administrated intraperitoneally and thereafter retinal morphology and functions were analyzed. In addition, we performed DNA microarray analysis both in nilvadipine treated and control retinas to understand their drug effects at molecular levels. We found that nilvadipine caused significant preservation of retinal thickness in rd mouse during the initial stage of the retinal degeneration, and nicardipine showed also significant but lesser preservation than nilvadipine. However, we recognized no preservation effects of diltiazem and nifedipine. In the total 3774 genes, the expressions of 27 genes were altered upon administration of nilvadipine, including several genes related to the apoptotic pathway, neuro-survival factor, Ca2+ metabolisms, and other mechanisms. It is suggested that some types of Ca2+ channel blockers, such as nilvadipine and nicardipine, are able to preserve photoreceptor cells in rd mouse and can potentially be used to treat some RP patients.
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U2 - 10.1016/j.bbrc.2003.12.034
DO - 10.1016/j.bbrc.2003.12.034
M3 - Article
C2 - 14706644
AN - SCOPUS:9144222598
VL - 313
SP - 1015
EP - 1022
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -