Study for catecholamine-2′-deoxyguanosine adduct formation under biomimetic conditions using liquid chromatography-electrospray ionization-ion trap mass spectrometry

T. Oe, S. Matsumoto, T. Toyo'oka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The adduct formation of 2′-deoxyguanosine (dG) with L-adrenaline under biomimetic conditions (pH 7.5, 37°C) with or without oxidant (MnO2) was demonstrated in order to clarify the reaction mechanism and the structure. At least two adducts have been observed by liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-ion trap MS) and LC-photodiode array detection (LC-PAD) (compound a: more polar than dG, m/z 463 (M+H)+, λmax: 230, 320 nm; compound b: less polar than dG, m/z 445 (M+H)+, λmax: 220, 240, 320, 405 nm). Compound a appeared only in the early stage of the reaction prior to formation of compound b.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalJournal of Health Science
Volume47
Issue number4
DOIs
Publication statusPublished - 2001 Aug 23
Externally publishedYes

Keywords

  • 2′-deoxyguanosine
  • Adrenaline
  • Catecholamine
  • DNA adduct
  • Michael addition
  • Orthoquinone

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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