Flow cytometry is widely used to classify individual cells optically but blood sampling is required for cell measurement in blood. If the principle of the cytometry is applicable directly in vivo, diagnosis of malaria would become easier. However, optical observation of the cell in blood flow is difficult because absorption and scattering of the light is large in vivo. In this study, novel ultrasonic method called "Sono-cytometry" for differentiating cells is proposed. The backscatter signal from the sphere with the diameter of 8 and 10-micron were detected by a transducer with the central frequency of 75 MHz. When the theoretical value is calculated from Faran-Hickling Model, linear approximate slope matched with theoretical slope. When calculating the slope of theoretical value by changing the spherical diameter, the slope value was very different between 8 and 10 micron. The result shows that it is possible that this method can classify spheres.
|Journal||Transactions of Japanese Society for Medical and Biological Engineering|
|Publication status||Published - 2014 Aug 17|
- High frequency ultrasound
- Micro fluidics
ASJC Scopus subject areas
- Biomedical Engineering