Studies on sandwich culture by making use of biofunctional hydrogels as a three-dimensional culture environment

Masaya Yamamoto, Hiroyuki Toda, Yasuhiko Tabata

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of this study is to obtain the fundamental knowledge of mesenchymal stem cells (MSC) cultured in a sandwiched condition with biofunctional hydrogels as a 3-D culture model. The biofunctional hydrogels were prepared through the immobilization of a cell signaling protein on poly(acrylamide) hydrogels with different crosslinking densities. As the cell signaling protein, ephrinB2 of an Eph signal ligand was employed to stimulate the osteoblastic differentiation of MSC. In this study, by making use of the specific interaction between IgG Fc domain and proteinA, the immobilization of ephrinB2-Fc in an orientation-regulated manner was designed to achieve the Eph signaling.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalKOBUNSHI RONBUNSHU
Volume75
Issue number1
DOIs
Publication statusPublished - 2018 Jan

Keywords

  • Biofunctional hydrogel
  • Orientation-regulated protein immobilization
  • Sandwich culture
  • Stem cell
  • Tissue engineering

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

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