Studies on external electric field effects on absorption and fluorescence spectra of NADH

Takakazu Nakabayashi, Md Serajul Islam, Liming Li, Masahide Yasuda, Nobuhiro Ohta

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Electric field effects on absorption and fluorescence spectra have been investigated for NADH that is a representative autofluorescent chromophore in cells. The change in electric dipole moment following absorption is significant in the electroabsorption spectrum, indicating charge transfer character in the excited state. The fluorescence intensity decreases in the presence of an electric field, which arises from the field-induced increase in the rate of the non-radiative process. The blue shift of the fluorescence spectrum and the increase in the fluorescence lifetime of NADH are measured in yeast cells, which is discussed in terms of a local electric field around NADH.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalChemical Physics Letters
Volume595-596
DOIs
Publication statusPublished - 2014 Mar 18
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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