Studies of ovomucoid-, avidin-, conalbumin- and flavoprotein-conjugated chiral stationary phases for separation of enantiomers by high-performance liquid chromatography

Nariyasu Mano, Yoshiya Oda, Naoki Asakawa, Yutaka Yoshida, Tadashi Sato, Toshinobu Miwa

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The effects of organic modifiers, buffer salts and pH on the retention and chiral separation of four protein-immobilized chiral stationary phases (CSPs), an ovomucoid-CSP, an avidin-CSP, a conalbumin-CSP and a flavoprotein-CSP, were investigated. Both retention and enantioselectivity were affected by alteration of the mobile phase conditions, and it was elucidated that the hydrophobic and ionic interactions between enantiomers and chiral recognition moieties in immobilized protein molecules were important to the chiral separation of each CSP. The protein bindings of enantiomers for four native proteins were also examined with chiral separation chromatography using a commercial ovomucoid-CSP (Ultron ES-OVM). The racemate which showed significant differences in protein binding abilities among its enantiomers was excellently resolved by chromatography.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalJournal of Chromatography A
Volume687
Issue number2
DOIs
Publication statusPublished - 1994 Dec 23
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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