Structures of primer-template hybrids in arbitrarily primed polymerase chain reaction

Keita Kawakami, Jun Yasuda, Takamasa Kayama, Katsuhiko Doi, Takao Sekiyaa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Nucleotide sequence analysis of arbitrarily primed PCR products from two known regions of human genome revealed that at least six contiguous bases at the 3'-end of a primer of 20 bases were perfectly matched in the primer- template hybrids.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalGenetic Analysis - Biomolecular Engineering
Volume15
Issue number1
DOIs
Publication statusPublished - 1999 Mar

Keywords

  • AP-PCR
  • DNA fingerprinting
  • Primer-template hybrid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

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