TY - JOUR
T1 - Structures of genomic and complementary DNAs coding for Pleurotus ostreatus manganese (II) peroxidase
AU - Asada, Yasuhiko
AU - Watanabe, Akira
AU - Irie, Toshikazu
AU - Nakayama, Toru
AU - Kuwahara, Masaaki
N1 - Funding Information:
This work was supporteidn part by a Grant-in-Aidfo r ScientificR esearchC from the Ministry of Education, Sciencea, nd Cultureo f Japan and by a grantf rom Novo NordiskB ioindustry.
PY - 1995/9/6
Y1 - 1995/9/6
N2 - To study the mechanism of regulation and structure/function relationship of the Pleurotus ostreatus manganese (11) peroxidase (MnP), we amplified the full-length genomic and complementary DNAs for the major isozyme of the MnP mainly by the cassette-primer PCR technique and then sequenced them. The cDNA contained an open reading frame of 1083 by encoding for a polypeptide of 361 amino-acid residues, including the suggested signal peptide of 29 amino-acid residues with a prepro structure. The predicted amino-acid sequence of the protein shared several common characteristics with those of fungal lignin and manganese (11) peroxidases. We could find a suggested metal response element and two heat-shock element-like sequences in the 5′-flanking region of the structural gene. The structural gene contained 15 introns, many of which lie identical to those in lignin peroxidase genes rather than to those in the known MnP genes.
AB - To study the mechanism of regulation and structure/function relationship of the Pleurotus ostreatus manganese (11) peroxidase (MnP), we amplified the full-length genomic and complementary DNAs for the major isozyme of the MnP mainly by the cassette-primer PCR technique and then sequenced them. The cDNA contained an open reading frame of 1083 by encoding for a polypeptide of 361 amino-acid residues, including the suggested signal peptide of 29 amino-acid residues with a prepro structure. The predicted amino-acid sequence of the protein shared several common characteristics with those of fungal lignin and manganese (11) peroxidases. We could find a suggested metal response element and two heat-shock element-like sequences in the 5′-flanking region of the structural gene. The structural gene contained 15 introns, many of which lie identical to those in lignin peroxidase genes rather than to those in the known MnP genes.
KW - (P. ostreatus)
KW - Lignin degradation
KW - Lignin peroxidase
KW - Manganese (11) peroxidase
KW - White-rot basidiomycete
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U2 - 10.1016/0167-4838(95)00102-Z
DO - 10.1016/0167-4838(95)00102-Z
M3 - Article
C2 - 7669812
AN - SCOPUS:0029111892
VL - 1251
SP - 205
EP - 209
JO - BBA - Protein Structure
JF - BBA - Protein Structure
SN - 1570-9639
IS - 2
ER -