Structures of genomic and complementary DNAs coding for Pleurotus ostreatus manganese (II) peroxidase

Yasuhiko Asada, Akira Watanabe, Toshikazu Irie, Toru Nakayama, Masaaki Kuwahara

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

To study the mechanism of regulation and structure/function relationship of the Pleurotus ostreatus manganese (11) peroxidase (MnP), we amplified the full-length genomic and complementary DNAs for the major isozyme of the MnP mainly by the cassette-primer PCR technique and then sequenced them. The cDNA contained an open reading frame of 1083 by encoding for a polypeptide of 361 amino-acid residues, including the suggested signal peptide of 29 amino-acid residues with a prepro structure. The predicted amino-acid sequence of the protein shared several common characteristics with those of fungal lignin and manganese (11) peroxidases. We could find a suggested metal response element and two heat-shock element-like sequences in the 5′-flanking region of the structural gene. The structural gene contained 15 introns, many of which lie identical to those in lignin peroxidase genes rather than to those in the known MnP genes.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1251
Issue number2
DOIs
Publication statusPublished - 1995 Sep 6
Externally publishedYes

Keywords

  • (P. ostreatus)
  • Lignin degradation
  • Lignin peroxidase
  • Manganese (11) peroxidase
  • White-rot basidiomycete

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Structures of genomic and complementary DNAs coding for Pleurotus ostreatus manganese (II) peroxidase'. Together they form a unique fingerprint.

  • Cite this