Structure of the snake short‐chain neurotoxin, erabutoxin c, precursor gene

Naoyuki FUSE, Takahide TSUCHIYA, Yoshiaki NONOMURA, Andre MENEZ, Toru TAMIYA

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

A 2.4‐kb genomic clone, which encodes the precursor of a snake neurotoxin, erabutoxin c, was isolated from the liver of the sea snake, Laticauda semifasciata. The erabutoxin c gene is about 1.2 kb long and consists of three exons and two introns. The first intron is found at the position corresponding to the signal peptide between amino acid residues 18(Leu) and 19(Gly). The second intron is located at the position corresponding to the central loop of the mature toxin, between amino acid residues 33(Arg) and 34(Gly). A TATA box consensus sequence, characteristic of promoter regions, is identified 29–33 nucleotides upstream from the transcription‐initiation site identified by nuclease S1 analysis. The erabutoxin c cDNA nucleotide sequence, deduced from the present work, is compared with the cDNA sequences of erabutoxins a and b reported previously. Replacements are found at three positions, two of which correspond to the amino acid replacements found among the toxins, while the other is silent.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalEuropean Journal of Biochemistry
Volume193
Issue number3
DOIs
Publication statusPublished - 1990 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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