Structure of the Rotor of the Bacterial Flagellar Motor Revealed by Electron Cryomicroscopy and Single-particle Image Analysis

Hirofumi Suzuki, Koji Yonekura, Keiichi Namba

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

The FliF ring is the base for self-assembly of the bacterial flagellum and the FliF/FliG ring complex is the core of the rotor of the flagellar motor. We report the structures of these two ring complexes obtained by electron cryomicroscopy and single-particle image analysis at 22Å and 25Å resolution, respectively. Direct comparison of these structures with the flagellar basal body made by superimposing the density maps on the central section reveals many interesting features, such as how the mechanically stable connection between the ring and the rod is formed, how directly FliF domains are involved in the near axial density of the basal body forming the proximal end of the central channel for a potential gating mechanism, some indication of flexibility in the connection of FliF and FliG, and structural and functional similarities to the head-to-tail connectors of bacteriophages.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalJournal of Molecular Biology
Volume337
Issue number1
DOIs
Publication statusPublished - 2004 Mar 12
Externally publishedYes

Keywords

  • Bacterial flagellum
  • Basal body
  • Electron cryomicroscopy
  • Flagellar motor
  • Single particle image analysis

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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