Structure of the murine secretory leukoprotease inhibitor (Slpi) gene and chromosomal localization of the human and murine SLPI genes

Toshiaki Kikuchi, Tatsuya Abe, Sachiko Hoshi, Nobumichi Matsubara, Yasuyuki Tominaga, Ken Satoh, Toshihiro Nukiwa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Secretory leukoprotease inhibitor (SLPI) is a serine protease inhibitor involved in antineutrophil elastase protection at inflammatory sites. To elucidate both the function and regulation of SLPI in vivo, we isolated and characterized the mouse Slpi gene. An entire 3-kb mouse Slpi gene fragment was sequenced, including an 0.8-kb 5′-flanking region, the 2.2-kb Slpi gene, and a 0.1-kb 3′-flanking region. The mouse Slpi gene spans 2,222 base pairs containing four exons and three introns. All splicing borders between exons and introns are conserved as predicted by GT-AG rules. Using primer extension analysis, the transcription start site was located 20 nucleotides upstream from the methionine (ATG) initiation codon. At the defined transcription start site, the sequence TCA+lGAGC is present. These results indicate that both mouse and human genomic structure are highly conserved. Using fluorescence in situ hybridization, we confirmed that, consistent with the genomic similarity, the human SLPI gene is localized on chromosome 20q 12-13.2 and the mouse homologue on chromosome 2H, which are syntenic with each other.

Original languageEnglish
Pages (from-to)875-880
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume19
Issue number6
DOIs
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Structure of the murine secretory leukoprotease inhibitor (Slpi) gene and chromosomal localization of the human and murine SLPI genes'. Together they form a unique fingerprint.

Cite this