Structure of the L-histidine decarboxylase gene

Kimio Yatsunami, Hiroshi Ohtsu, Motoko Tsuchikawa, Toshio Higuchi, Kaname Ishibashi, Ayumi Shida, Youichiroh Shima, Satoshi Nakagawa, Kohei Yamauchi, Masayuki Yamamoto, Norio Hayashi, Takehiko Watanabe, Atsushi Ichikawa

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

Two species of L-histidine decarboxylase (HDC) mRNA were found in the KU- 812-F basophilic cell line, but only the 2.4-kilobase (kb) one encodes the functional HDC (Mamune-Sato, R., Yamauchi, K., Tanno, Y., Ohkawara, Y., Ohtsu, H., Katayose, D., Maeyama, K., Watanabe, T., Shibahara, S., and Takishima, T. (1992) Eur. J. Biochem. 209, 533-539). The 3.4-kb one encodes a truncated HDC protein and is also found in human leukemia-derived cell lines HEL and KCL-22. To clarify the mechanisms that regulate transcription of the HDC gene and generate the two species of mRNA, we have isolated genomic DNA clones coding for the HDC from human genomic libraries. Structural analysis of the isolated clones revealed that the human HDC gene is composed of 12 exons spanning approximately 24 kb. Genomic DNA blot analysis suggested that HDC is encoded by a single copy gene. The structural analysis also demonstrated that the heterogeneity of the HDC mRNA is caused by an insertion of the seventh intron sequence and alternative use of the splicing acceptor site at the 12th exon. The transcription start site of the HDC gene and the nucleotide sequences of the promoter and first exon regions were determined. We found a TATA-like sequence, a GC box, four CACC boxes, four GATA consensus sequences, and six leader-binding protein-1 binding motifs in the promoter region of the HDC gene.

Original languageEnglish
Pages (from-to)1554-1559
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number2
Publication statusPublished - 1994 Jan 14

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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