Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction

Yusuke Akai, Naruhiko Adachi, Yohei Hayashi, Masamitsu Eitoku, Norihiko Sano, Ryo Natsume, Norio Kudo, Masaru Tanokura, Toshiya Senda, Masami Horikoshi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nucleosomes around the promoter region are disassembled for transcription in response to various signals, such as acetylation and methylation of histones. Although the interactions between histone-acetylation-recognizing bromodomains and factors involved in nucleosome disassembly have been reported, no structural basis connecting histone modifications and nucleosome disassembly has been obtained. Here, we determined at 3.3 Å resolution the crystal structure of histone chaperone cell cycle gene 1 (CCG1) interacting factor A/antisilencing function 1 (CIA/ASF1) in complex with the double bromodomain in the CCG1/TAF1/TAF(II)250 subunit of transcription factor IID. Structural, biochemical, and biological studies suggested that interaction between double bromodomain and CIA/ASF1 is required for their colocalization, histone eviction, and pol II entry at active promoter regions. Furthermore, the present crystal structure has characteristics that can connect histone acetylation and CIA/ASF1-mediated histone eviction. These findings suggest that the molecular complex between CIA/ASF1 and the double bromodomain plays a key role in site-specific histone eviction at active promoter regions. The model we propose here is the initial structure-based model of the biological signaling fromhistone modifications to structural change of the nucleosome (hi-MOST model).

Original languageEnglish
Pages (from-to)8153-8158
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number18
DOIs
Publication statusPublished - 2010 May 4

Keywords

  • Chromatin
  • Transcription
  • Transcription factor IID
  • X-ray crystallography

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction'. Together they form a unique fingerprint.

  • Cite this