Structure of human cholecystokinin gene and its chromosomal location

Takahashi Yoosuke; Fukushige Shinichi; Murotsu Tomoaki; Matsubara Kenichi

doi:10.1016/0378-1119(86)90339-2

Structure of human cholecystokinin gene and its chromosomal location

Takahashi Yoosuke, Fukushige Shinichi, Murotsu Tomoaki, Matsubara Kenichi

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

We have determined the entire structure of human cholecystokinin (CCK) gene, which is 7 kb in size and separated into three exons. S1 endonuclease analysis has shown two putative transcription initiation sites that are preceded by 'TATA' equivalent sequences located 39 bp and 35 bp upstream from these sites. The promoter region contains five 'G-C box'-like sequences, which are believed to be spl-binding sites. By chromosome sorting in combination with velocity sedimentation and Southern hybridization, the human cck gene was mapped on the short arm of human chromosome 3.

Original language	English
Pages (from-to)	353-360
Number of pages	8
Journal	Gene
Volume	50
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(86)90339-2
Publication status	Published - 1986
Externally published	Yes

Keywords

Recombinant DNA
S1 mapping
cell sorter
exon
flanking sequence
gastrin gene

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(86)90339-2

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title = "Structure of human cholecystokinin gene and its chromosomal location",

abstract = "We have determined the entire structure of human cholecystokinin (CCK) gene, which is 7 kb in size and separated into three exons. S1 endonuclease analysis has shown two putative transcription initiation sites that are preceded by 'TATA' equivalent sequences located 39 bp and 35 bp upstream from these sites. The promoter region contains five 'G-C box'-like sequences, which are believed to be spl-binding sites. By chromosome sorting in combination with velocity sedimentation and Southern hybridization, the human cck gene was mapped on the short arm of human chromosome 3.",

keywords = "Recombinant DNA, S1 mapping, cell sorter, exon, flanking sequence, gastrin gene",

author = "Takahashi Yoosuke and Fukushige Shinichi and Murotsu Tomoaki and Matsubara Kenichi",

note = "Funding Information: This study was supportedb y grants from the Ministry of Education, Science, and Culture of Japan.",

year = "1986",

doi = "10.1016/0378-1119(86)90339-2",

language = "English",

volume = "50",

pages = "353--360",

journal = "Gene",

issn = "0378-1119",

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TY - JOUR

T1 - Structure of human cholecystokinin gene and its chromosomal location

AU - Yoosuke, Takahashi

AU - Shinichi, Fukushige

AU - Tomoaki, Murotsu

AU - Kenichi, Matsubara

N1 - Funding Information: This study was supportedb y grants from the Ministry of Education, Science, and Culture of Japan.

PY - 1986

Y1 - 1986

N2 - We have determined the entire structure of human cholecystokinin (CCK) gene, which is 7 kb in size and separated into three exons. S1 endonuclease analysis has shown two putative transcription initiation sites that are preceded by 'TATA' equivalent sequences located 39 bp and 35 bp upstream from these sites. The promoter region contains five 'G-C box'-like sequences, which are believed to be spl-binding sites. By chromosome sorting in combination with velocity sedimentation and Southern hybridization, the human cck gene was mapped on the short arm of human chromosome 3.

AB - We have determined the entire structure of human cholecystokinin (CCK) gene, which is 7 kb in size and separated into three exons. S1 endonuclease analysis has shown two putative transcription initiation sites that are preceded by 'TATA' equivalent sequences located 39 bp and 35 bp upstream from these sites. The promoter region contains five 'G-C box'-like sequences, which are believed to be spl-binding sites. By chromosome sorting in combination with velocity sedimentation and Southern hybridization, the human cck gene was mapped on the short arm of human chromosome 3.

KW - Recombinant DNA

KW - S1 mapping

KW - cell sorter

KW - exon

KW - flanking sequence

KW - gastrin gene

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DO - 10.1016/0378-1119(86)90339-2

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AN - SCOPUS:0022909166

SN - 0378-1119

VL - 50

SP - 353

EP - 360

JO - Gene

JF - Gene

IS - 1-3

ER -

Structure of human cholecystokinin gene and its chromosomal location

Abstract

Keywords

ASJC Scopus subject areas

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