Structure of human cholecystokinin gene and its chromosomal location

Takahashi Yoosuke, Fukushige Shinichi, Murotsu Tomoaki, Matsubara Kenichi

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

We have determined the entire structure of human cholecystokinin (CCK) gene, which is 7 kb in size and separated into three exons. S1 endonuclease analysis has shown two putative transcription initiation sites that are preceded by 'TATA' equivalent sequences located 39 bp and 35 bp upstream from these sites. The promoter region contains five 'G-C box'-like sequences, which are believed to be spl-binding sites. By chromosome sorting in combination with velocity sedimentation and Southern hybridization, the human cck gene was mapped on the short arm of human chromosome 3.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalGene
Volume50
Issue number1-3
DOIs
Publication statusPublished - 1986
Externally publishedYes

Keywords

  • Recombinant DNA
  • S1 mapping
  • cell sorter
  • exon
  • flanking sequence
  • gastrin gene

ASJC Scopus subject areas

  • Genetics

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