Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon

Masahiro Sota, Haruhiko Kawasaki, Masataka Tsuda

    Research output: Contribution to journalArticlepeer-review

    47 Citations (Scopus)

    Abstract

    The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, TnHad1 and TnHad2, and the mer genes for resistance to mercury. The complete 67,066-bp sequence of pUO1 revealed that the mer genes were also carried by two Tn402/Tn5053-like transposons, Tn4671 and Tn4672, and that the pUO1 backbone regions shared 99% identity to those of the archetype IncP-1β plasmid R751. Comparison of pUO1 with three other IncP-1β plasmids illustrated the importance of transposon insertion in the diversity and evolution of this group of plasmids. Mutational analysis of the four outermost residues in the inverted repeats (IRs) of TnHad2, a Tn21-related transposon, revealed a crucial role of the second residue of its IRs in transposition.

    Original languageEnglish
    Pages (from-to)6741-6745
    Number of pages5
    JournalJournal of bacteriology
    Volume185
    Issue number22
    DOIs
    Publication statusPublished - 2003 Nov

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

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