Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

Koji Yonekura, Chikashi Toyoshima

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)15-28
Number of pages14
JournalUltramicroscopy
Volume84
Issue number1-2
DOIs
Publication statusPublished - 2000 Jul
Externally publishedYes

Keywords

  • Ca-ATPase
  • Cryo-electron microscopy
  • Real-space averaging
  • Tubular crystal

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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